Involvement of protein kinase in Δ12‐prostaglandin J2‐induced expression of rat heme oxygenase‐1 gene
- 25 September 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 372 (2-3), 279-282
- https://doi.org/10.1016/0014-5793(95)01001-u
Abstract
We recently identified the cis-regulatory element and its specific nuclear binding factors for Δ12-prostaglandin (PG) J2-induced expression of the rat heme oxygenase, HO-1 [Koizumi, T., Odani, N., Okuyama, T., Ichikawa, A. and Negishi, M. (1995)J. Biol. Chem. 270, in press]. Here we further examined the molecular mechanism underlying the Δ12-PGJ2-induced HO-1 gene expression. Protein kinase inhibitors, 2-aminopurine and staurosporine, suppressed the Δ12-PGJ2-induced HO-1 mRNA and the nuclear protein binding to the Δ12-PGJ2-responsivecis-regulatory element in rat basophilic leukemia cells. Furthermore, the nuclear protein binding to the element was suppressed by in vitro phosphatase treatment of the nuclear proteins from Δ12-PGJ2-treated cells. These findings suggest that Δ12-PGJ2 induces the expression of the HO-1 gene through phosphorylation of the nuclear proteins which bind to the Δ12-PGJ2-responsive elementKeywords
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