A scheme for detection of metabolic disorders utilizing commercial dip tests, spot plate tests, and paper chromatographic tests is presented. Specific details are given for preparation and development of chromatograms for routine screening of urine specimens for disorders of amino acid and carbohydrate metabolism. Specialized tests for confirming positive findings in the screening procedures are described. The results are interpreted with regard to the variations encountered in testing normal infants and children, children hospitalized with a variety of diseases, and mentally retarded children. Examples of specific and generalized aminoacidurias are given.