The effect of reduction of perfusion rate on lactate and oxygen uptake, glucose output and energy supply in the isolated perfused liver of starved rats
- 15 December 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 184 (3), 635-642
- https://doi.org/10.1042/bj1840635
Abstract
Lactate and O2 uptake and glucose output were studied in isolated livers from starved rats at perfusate flow rates varying from 100-7% of normal (11.25-0.75 ml/min per 100 g body wt.). With moderate diminution of flow rate, lactate and O2 uptake fell more slowly than would be expected if uptake purely depended on substrate supply. Use of a mathematical model suggests that the intrinsic capacity of the liver for lactate uptake is unaffected until the flow rate falls below 25% of normal. Some lactate uptake was always observed even at 7% of the normal flow rate. At flow rates below 33% of the normal, lactate was increasingly metabolized by pathways other than gluconeogenesis, which became a progressively less important consumer of available O2. ATP content decreased with diminution of flow rate, but substantially less markedly than did lactate uptake and glucose output. Intracellular pH fell from a mean value of 7.25 at normal flow rate to 7.03 at 7% of the normal flow rate.This publication has 14 references indexed in Scilit:
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