Pepsin Fragments of Human Placental Basement-Membrane Collagens Showing Interrupted Triple-Helical Amino Acid Sequences
- 1 January 1981
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 362 (2), 943-952
- https://doi.org/10.1515/bchm2.1981.362.2.943
Abstract
The pepsin digestion of human placental type IV collagen was studied to gain insight into the relationships between the fragments .alpha.1(IV)95,000 and .alpha.2(IV)70,000 which were previously described. Seven smaller pepsin fragments with apparent MW of 5000-50,000 were also isolated and characterized. The amino-terminal amino acid sequences of .alpha.1(IV)95,000, .alpha.2(IV)70,000 and the smaller pepsin fragments are presented. Kinetic data on the pepsin solubilization provided evidence for the presence of at least 2 different collagen triple helices within basement membranes and sequence data demonstrated the presence of a number of short non-collagen-like sequences which interrupt the triple helical regions of the .alpha. chains.This publication has 19 references indexed in Scilit:
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