P700 Chlorophyll a-Protein
Open Access
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 72 (3), 625-633
- https://doi.org/10.1104/pp.72.3.625
Abstract
The P700 chlorophyll α-protein was purified by preparative sodium dodecyl sulfate (SDS) gel electrophoresis from SDS-solubilized barley (Hordeum vulgare L., cv Himalaya) chloroplast membranes. After elution from the gel in the presence of 0.05 to 0.1% Triton X-100, the recovered protein had a chlorophyll/P700 ratio of 50 to 60/1 and contained no chlorophyll b or cytochromes. Analysis of the polypeptide composition of the chlorophyll-protein revealed a 58 to 62 kilodalton (kD) polypeptide component but no lower molecular weight polypeptides. The 58 to 62 kD component was further resolved into two distinct polypeptide bands which were subsequently mapped by partial cyanogen bromide digestion and Staphylococcus aureus proteolysis. Based on results from the mapping experiments and other data, we suggest that the two components are conformational variants of a single polypeptide. Measurement of the chlorophyll to protein ratio by quantitative amino acid analysis and consideration of the yield of P700 in the protein isolate suggest that, contrary to previous models (Bengis and Nelson, 1975, 1977), P700in vivo is associated with a minimum of four subunits of approximately 60 kD.This publication has 23 references indexed in Scilit:
- P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoidsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- ELECTROPHORETIC AND SPECTROPHOTOMETRIC STUDIES OF CHLOROPHYLL‐PROTEIN COMPLEXES FROM TOBACCO CHLOROPLASTS. ISOLATION OF A LIGHT HARVESTING PIGMENT PROTEIN COMPLEX WITH A MOLECULAR WEIGHT OF 70,000Photochemistry and Photobiology, 1977
- A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii.The Journal of cell biology, 1975
- The relationship between chlorophyll-protein complexes and chloroplast membrane polypeptidesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1974
- The P700-chlorophyll a-protein of a blue-green algaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Comparison of a chlorophyll a-protein complex isolated from a blue-green alga with chlorophyll-protein complexes obtained from green bacteria and higher plantsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1969
- Two pigment proteins in spinach chloroplastsBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949