Synthesis of porcine neuropeptide Y(NPY) in solution

Abstract

The porcine neuropeptide Y (NPY), a 36-residue peptide amide, was synthesized by assembling six peptide fragments followed by thioanisole-mediated deprotection with trifluoromethanesulfonic acid in trifluoroacetic acid. β-Cycloheptyl aspartate, Asp(OChp), was employed to suppress base-catalyzed succinimide formation. When administered to dogs, the purified peptide (10μg/kg) caused prolonged increase of systemic arterial blood pressure and decreased pancreatic blood flow.