ISOLATION AND PARTIAL CHARACTERIZATION OF A GENUS COMMON ANTIGEN AND SPECIES SPECIFIC ANTIGEN OF CAPNOCYTOPHAGA
- 1 January 1982
- journal article
- research article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 35 (4), 153-170
- https://doi.org/10.7883/yoken1952.35.153
Abstract
Sixteen strains of Capnocytophaga were isolated from the pocket of a localized juvenile periodonititis patient. These strains were divied into 4 groups on the basis of morphological and physiological traits. Strains from group I and group III were identified as C. ochracea and group II as C. sputigena. An antigen common to genus Capnocytophaga was purified using immunoabsorbent chromatography from lysates obtained by sodium dodecyl sulfate treatment of C. ochracea strain S1. An antigen specific to C. ochracea was prepared by sequential gel filtration and preparative isoelectric focusing. The genus common and species specific antigens isolated were immunologically unique and pure when tested by immunoelectrophoresis and immunodiffusion against rabbit antisera prepared to Capnocytophaga and other gram-negative rods. The genus common antigen was susceptible to trypsin and pronase digestion, was soluble in chloroform-methanol, but was unaltered by RNase and DNase treatments and periodate oxidation. Antigenicity of the species specific antigen was destroyed by periodate oxidation. The genus common antigen appeared to be lipid-associated protein, while the species specific antigen consisted mainly of carbohydrate. These specific immunological reagents would be valuable in diagnosing and monitoring diseases.This publication has 16 references indexed in Scilit:
- Capnocytophaga: New genus of gram-negative gliding bacteria I. General characteristics, taxonomic considerations and significanceArchiv für Mikrobiologie, 1979
- Histological changes in experimental periodontal disease in rats monoinfacted with gram-negative organismsJournal of Periodontal Research, 1978
- Multiple bands produced by interaction of a single macromolecule with carrier ampholytes during isoelectric focusingArchives of Biochemistry and Biophysics, 1978
- Predominant cultivable microbiota in periodontosisJournal of Periodontal Research, 1977
- Studies of the Microbiology of PeriodontosisThe Journal of Periodontology, 1976
- Immuno-electrophoretic and chemical analyses of human parotid salivaArchives of Oral Biology, 1973
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Lysis of the cell membrane of Escherichia coli K12 by ionic detergentsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- A simplified spectrophotometric determination of ester groups in lipidsBiochimica et Biophysica Acta, 1959