1H,13C and15N backbone assignments of cyclophilin when bound to cyclosporin A (CsA) and preliminary structural characterization of the CsA binding site
- 2 December 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 294 (1-2), 81-88
- https://doi.org/10.1016/0014-5793(91)81348-c
Abstract
The backbone 1H, 13C and 15N chemical shifts of cyclophilin (CyP) when bound to cyclosporin A (CsA) have been assigned from heteronuclear two- and three-dimensional NMR experiments involving selectively 15N- and uniformly 15N- and 15N,13C-labeled cyclophilin. From an analysis of the 1H and 15N chemical shifts of CyP that change upon binding to CsA and from CyP/CsA NOEs, we have determined the regions of cyclophilin involved in binding to CsAKeywords
This publication has 30 references indexed in Scilit:
- Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexesCell, 1991
- Protein secondary structure determination by NMR Application with recombinant human cyclophilinFEBS Letters, 1991
- An efficient 3D NMR technique for correlating the proton and15N backbone amide resonances with the α-carbon of the preceding residue in uniformly15N/13C enriched proteinsJournal of Biomolecular NMR, 1991
- Chemistry and Biology of the Immunophilins and Their Immunosuppressive LigandsScience, 1991
- Four-dimensional [13C,1H,13C,1H] HMQC-NOE-HMQC NMR spectroscopy: resolving tertiary nuclear Overhauser effect distance constraints in the spectra of larger proteinsJournal of the American Chemical Society, 1991
- Assignment of the backbone proton and nitrogen-15 NMR resonances of bacteriophage T4 lysozymeBiochemistry, 1990
- Heteronuclear three-dimensional NMR spectroscopy of isotopically labelled biological macromoleculesQuarterly Reviews of Biophysics, 1990
- Clean TOCSY for proton spin system identification in macromoleculesJournal of the American Chemical Society, 1988
- NMR signal assignments of amide protons in the .alpha.-helical domains of staphylococcal nucleaseBiochemistry, 1988
- Cyclophilin: A Specific Cytosolic Binding Protein for Cyclosporin AScience, 1984