Adenine and Guanine Salvage in Suspension Cultured Cells of Catharanthus roseus*

Abstract
Changes in the activity of adenine and guanine salvage in nucleotide and nucleic acid synthesis during the growth of Catharanthus roseus were investigated. Incorporation of [8-14C]adenine into ATP and ADP and that of [8-14C]guanine into GTP and GDP increased markedly in the lag phase of cell growth and then sharply decreased. The incorporation into RNA from both precursors showed a similar pattern. The role of rapid purine salvage observed in the lag phase of cell growth is discussed.