A quantitative chromatographic method for the determination of purity of oxytetracycline
- 1 December 1969
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 21 (Supplement), 40S-43S
- https://doi.org/10.1111/j.2042-7158.1969.tb08345.x
Abstract
A chromatographic method for the determination of oxytetracycline is described, employing a hexane-ethyl acetate-pH 3·3 buffer partition system supported on Celite. Chromatographic separation is followed by measuring the absorbance of the eluate at 263 nm. The system enables the determination of oxytetracycline to be made in the presence of anhydro-oxytetracycline, chlortetracycline, α- and β-apo-oxytetracycline, and epi-oxytetracycline, the last three being the most likely contaminants of crude material.Keywords
This publication has 1 reference indexed in Scilit:
- Terramycin: Chemical methods of assay and identification**Analytical Laboratories Chas. Pfizer & Co., Inc., Brookly, N.Y.Journal of the American Pharmaceutical Association (Scientific ed.), 1951