Mapping of Cleavage Sites for Restriction Endonucleases in λdv Plasmids

Abstract

Restriction nucleases from Escherichia coli carrying the resistance transfer factor RI, from Hemophilus influenzae, Hemophilus parainfluenzae, and Bacillus subtilis were used for the generation of specific DNA fragments from lambdadv plasmids. Cleavage maps were established for various plasmids containing different but overlapping parts of bacteriophage lambdaDNA by analysis of digestion patterns obtained in polyacrylamide gel electrophoresis. The correlation between the plasmid cleavage maps and the genetic map of lambda was based upon the location of the EcoRI cleavage site at 81.3% of lambda fractional length and the position of promoters PL and PR on the plasmid DNA fragments.