Simultaneous Determination of Caffeine and Ergotamine in Pharmaceutical Dosage Formulation by Capillary Electrophoresis

Abstract

A method has been developed for the simultaneous assay of caffeine and ergotamine in the pharmaceutical dosage tablet formulations (Cafergot_®) by capillary electrophoresis (CE). The analysis was accomplished by using 25 mM sodium phosphate buffer at pH 6.0 with UV detection at 214 nm. The mean recoveries were 98.9% ± 1.9% and 101% ± 1.03% for the analysis of caffeine and ergotamine respectively. Run time was less than six minutes for the analysis of the two active constituents in the tablets. There were no excipients interferences.