Formation of recombinants between snowshoe hare and La Crosse bunyaviruses
- 1 December 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 24 (3), 893-902
- https://doi.org/10.1128/jvi.24.3.893-902.1977
Abstract
Wild-type recombinants were obtained at high frequency from co-infections of BHK [baby hamster kidney] cells involving temperature-sensitive, conditional-lethal mutants of snowshoe hare (SSH) and La Crosse (LAC) bunyaviruses. Analyses of 2 of the recombinants indicated that they have the genome compositions SSH/LAC/SSH and SSH/LAC/LAC for their respective L, M and S virion RNA species. This evidence, together with that for the genetic stability of the recombinants, indicates that they were derived by segment re-assortment of the competent genome pieces of the parental viruses. The SSH/LAC/SSH recombinant appears, from polypeptide analysis, to have the SSH type of nucleocapsid protein (N), whereas the SSH/LAC/LAC recombinant has the LAC nucleocapsid protein, suggesting that the viral S RNA codes for the N protein.This publication has 13 references indexed in Scilit:
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