Bacterial Profiles of Root Caries in Elderly Patients
- 1 June 2008
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (6), 2015-2021
- https://doi.org/10.1128/jcm.02411-07
Abstract
Culture-based studies have shown that Streptococcus mutans and lactobacilli are associated with root caries (RC). The purpose of the present study was to assess the bacterial diversity of RC in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. Plaque was collected from root surfaces of 10 control subjects with no RC and from 11 subjects with RC. The bacterial 16S rRNA genes from extracted DNA were PCR amplified, cloned, and sequenced to determine species identity. From a total of 3,544 clones, 245 predominant species or phylotypes were observed, representing eight bacterial phyla. The majority (54%) of the species detected have not yet been cultivated. Species of Selenomonas and Veillonella were common in all samples. The healthy microbiota included Fusobacterium nucleatum subsp. polymorphum, Leptotrichia spp., Selenomonas noxia, Streptococcus cristatus, and Kingella oralis. Lactobacilli were absent, S. mutans was present in one, and Actinomyces spp. were present in 50% of the controls. In contrast, the microbiota of the RC subjects was dominated by Actinomyces spp., lactobacilli, S. mutans, Enterococcus faecalis, Selenomonas sp. clone CS002, Atopobium and Olsenella spp., Prevotella multisaccharivorax, Pseudoramibacter alactolyticus, and Propionibacterium sp. strain FMA5. The bacterial profiles of RC showed considerable subject-to-subject variation, indicating that the microbial communities are more complex than previously presumed. The data suggest that putative etiological agents of RC include not only S. mutans, lactobacilli, and Actinomyces but also species of Atopobium, Olsenella, Pseudoramibacter, Propionibacterium, and Selenomonas.Keywords
This publication has 30 references indexed in Scilit:
- Genetic Profiling of the Oral Microbiota Associated with Severe Early-Childhood CariesJournal of Clinical Microbiology, 2007
- Oral microbial communities in sickness and in healthTrends in Microbiology, 2005
- Diversity of Bacterial Populations on the Tongue Dorsa of Patients with Halitosis and Healthy PatientsJournal of Clinical Microbiology, 2003
- TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environmentBioinformatics, 1994
- Effects of a 12-Month Prophylactic Programme on Selected Oral Bacterial Populations on Root Surfaces with Active and Inactive Carious LesionsCaries Research, 1993
- The association of selected bacteria with the lesions of root surface cariesOral Microbiology and Immunology, 1990
- Microflora of Plaque from Sound and Carious Root SurfacesCaries Research, 1987
- CLINICAL SCIENCE Longitudinal Microbiological Investigation of a Hospitalized Population of Older Adults with a High Root Surface Caries RiskJournal of Dental Research, 1985
- Streptococcus mutans and Lactobacillus Detection in the Assessment of Dental Root Surface Caries RiskJournal of Dental Research, 1985
- Characterization of Bacteria Isolated from Human Root Surface Carious LesionsJournal of Dental Research, 1974