Role of ascorbic acid on tyrosine hydroxylase activity in the adrenal gland of guinea pig.

Abstract

The decrease of tyrosine hydroxylase activity in adrenal homogenate in scurvy was recovered after the administration of ascorbic acid. The causes of the increase in the enzyme activity after the administration of ascorbic acid were studied. No significant elevation in the enzyme activity was observed after the administration of reserpine to the scorbutic guinea pig. A dose of metal chelating agent, .alpha., .alpha.''-dipyridyl, prevented the ascorbic acid-induced or reserpine-induced increase in enzyme activity in the scorbutic and the non-scorbutic guinea pigs, respectively. Tyrosine hydroxylase activity was partially recovered by the administration of FeSO4 to the scorbutic guinea pig. The induction of tyrosine hydroxylase which was not observed in scurvy was clearly due to the deficiency of Fe2+. Ascorbic acid probably affected the induction of this enzyme via Fe2+.