Metallothionein and copper in liver disease with copper retention—a histopathological study
- 1 June 1989
- journal article
- research article
- Published by Wiley in The Journal of Pathology
- Vol. 158 (2), 131-137
- https://doi.org/10.1002/path.1711580208
Abstract
We have examined the relationship between (a) histochemically demonstrable copper using rubeanic add, (b) copper-associated protein (CAP) using orcein, and (c) immunoreactive metallothionein (MT) using DNP hapten sandwich staining and have correlated these with histological lesions in 95 liver biopsies from patients with conditions associated with hepatic copper retention, 4 fetal livers, and 25 historically normal adult controls. No copper or CAP was present in normal adult liver but periportal CAP was present in fetal liver. MT was present in hepatocytes of normal livers with a predominantly perivenular (centrilobular) cytoplasmic distribution varying in staining intensify; all fetal hepatocytes stained strongly for MT. Fifty-two of 95 (55 per cent) abnormal livers contained CAP and 42 (44 per cent) contained both CAP and copper. In CAP-positive livers, the commonest histological lesions were piecemeal necrosis and cholestasis. CAP was present in (a) 15/15 cases of primary biliary cirrhosis including early cases with minimal pathology; and (b) 5/5 cases of Wilson's disease, 6/6 cases of biliary atresia, and 3/9 cases of sclerosing cholangitis. In other conditions, it was present in 25–50 per cent of cases. MT distribution was abnormal in most CAP-positive livers. Necrotic hepatocytes were intensely MT-positive and in Wilson's disease had a characteristic appearance.Keywords
This publication has 15 references indexed in Scilit:
- Sequential and diagnostic features in primary biliary cirrhosis based on serial histologic study in 209 patientsGastroenterology, 1985
- Hepatic copper distribution in primary biliary cirrhosis shown by the scanning proton microprobe.Journal of Clinical Pathology, 1985
- Histological demonstration of immunoreactive zinc metallothionein in liver and ileum of rat and manJournal of Molecular Histology, 1985
- Species differences in the occurrence of copper-metallothionein in the particulate fractions of the liver of copper-loaded animalsBiochemical Journal, 1984
- Value of copper-associated protein in diagnostic assessment of liver biopsy.Journal of Clinical Pathology, 1983
- BILIARY EXCRETION OF COPPER IN WILSON'S DISEASEThe Lancet, 1980
- Production and study of antibody produced aganist rat cadmium thioneinImmunochemistry, 1978
- Histological demonstration of copper and copper-associated protein in chronic liver diseases.Journal of Clinical Pathology, 1978
- Demonstration of an intracellular copper-binding protein by orcein staining in long-standing cholestatic liver diseases.Gut, 1976
- LIPOFUSCIN (AGING) PIGMENT GRANULES OF THE NEWBORN HUMAN LIVERThe Journal of cell biology, 1969