A Defined Medium for the Growth of the Thermophilic Actinomycete Micromonospora vulgaris

Abstract

Biotin and methionine are essential growth factors for the aerobic thermophilic actinomycete Micromonospora vulgaris. Biotin can be partially replaced by Tween 80 (sorbitan mono-oleate). No aerial growth is obtained in the absence of methionine. A chemically defined medium containing mineral salts, phosphate buffer (pH 6·8), soluble starch, a mixture of 18 synthetic amino acids (including methionine) and biotin will support growth of the organism.