Sequence-specific recognition of DNA: assignment of nonexchangeable proton resonances in the consensus Pribnow promoter DNA sequence by two-dimensional NMR
- 1 May 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (10), 2262-2268
- https://doi.org/10.1021/bi00305a027
Abstract
The resonances of most of the nonexchangeable protons of both + and - strands of the consensus Pribnow dodecamer d(CGTTATAATGCG) were assigned by 2-dimensional NMR methods. Application of the 2-dimensional nuclear Overhauser effect (NOESY) sequential connectivity method, combined with 2-dimensional autocorrelated (COSY) spectra to reveal scalar-coupled protons, results in assignment of virtually all of the base and sugar protons, except the sugar C5 protons which are inadequately resolved. Analysis of the nuclear Overhauser data indicates that the helix assumes a fairly uniform B form conformation.This publication has 4 references indexed in Scilit:
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- Proton nuclear magnetic resonance study of a self-complementary decadeoxyribonucleotide, C-C-A-A-G-C-T-T-G-GBiochemistry, 1982
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