Biochemical and Genetic Evidence for Phospholipase C Activity inMycobacterium ulcerans
- 1 May 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (5), 2995-7
- https://doi.org/10.1128/iai.68.5.2995-2997.2000
Abstract
This study reports the existence of phospholipase C and D enzymatic activities inMycobacterium ulceranscultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine.M. ulceransDNA sequences homologous to the genes encoding phospholipase C inMycobacterium tuberculosisandPseudomonas aeruginosawere identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes ofM. ulceransplays a role in the pathogenesis of the disease needs further investigation.Keywords
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