Preparation of Cell-Free Feline Oncornavirus-Associated Cell Membrane Antigen2

Abstract

Cell-free feline oncornavirus-associated cell membrane antigen (FOCMA) was prepared from a feline Iymphoblastoid cell line of tumor origin (FL-74). Membrane fractions, separated on sucrose density gradients, and papain-solubilized products were found to contain FOCMA as determined by their capacity to inhibit reference cytotoxic cat antisera.