Improved Polymerase Chain Reaction Technique for Determining the Species Composition of Eimeria in Poultry Litter
- 1 December 2006
- journal article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 50 (4), 632-635
- https://doi.org/10.1637/7615-042106r.1
Abstract
An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by cloning the hybrid DNA molecules into a plasmid. The internal DNA standards were then used in Eimeria-specific ITS 1 PCR, and they were found to be capable of detecting E. acervulina, E. maxima, E. praecox, and E. tenella oocysts isolated directly from poultry litter.Keywords
This publication has 17 references indexed in Scilit:
- Application of Polymerase Chain Reaction Based on ITS1 rDNA to Speciate EimeriaAvian Diseases, 2006
- High-throughput capillary electrophoresis for the identification and differentiation of seven species ofEimeria from chickensElectrophoresis, 2005
- Differential diagnosis of five avian Eimeria species by polymerase chain reaction using primers derived from the internal transcribed spacer 1 (ITS-1) sequenceVeterinary Parasitology, 2003
- A multiplex PCR assay for the simultaneous detection and discrimination of the sevenEimeriaspecies that infect domestic fowlParasitology, 2003
- A survey of the inter- and intraspecific RAPD markers of Eimeria spp. of the domestic fowl and the development of reliable diagnostic toolsZeitschrift Fur Parasitenkunde-Parasitology Research, 2002
- Anticoccidial vaccines for broiler chickens: Pathways to successAvian Pathology, 2002
- Eimeria parasites of domestic fowl: genetic relationships of different isolates estimated from random amplified polymorphic DNAZeitschrift Fur Parasitenkunde-Parasitology Research, 2001
- PCR identification of chicken Eimeria: A simplified read-outAvian Pathology, 1999
- Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic.Eimeriaspecies of the chickenAvian Pathology, 1998
- A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions.Genome Research, 1995