Substrate specificity of the lactate dehydrogenase isoenzyme C4 from human spermatozoa and a possible selective assay
- 1 January 1979
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 55 (1), 101-106
- https://doi.org/10.1530/jrf.0.0550101
Abstract
The activity of lactate dehydrogenase (EC 1.1.1.27) in normal human sperm lysates and in human heart and liver homogenates was determined by using a variety of 2-oxoacids as substrates. Sperm preparations were active with pyruvate, 2-oxobutanoate, 2-oxopentanoate and 2-oxohexanoate, while heart and liver extracts utilized only pyruvate and 2-oxobutanoate. Selective staining after gel electrophoresis indicated that the fraction corresponding to lactate dehydrogenase C4, the sperm-specific isoenzyme, was responsible for the utilization of substrates with a linear chain of 3-6 carbon atoms. The use of 5 mM 2-oxohexanoate allowed the selective determination of isoenzyme C4 in preparations containing different lactate dehydrogenase molecular forms.Keywords
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