Purification of a Growth Factor for Ovarian Cells from Bovine Pituitary Glands
- 1 June 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (6), 2295-2299
- https://doi.org/10.1073/pnas.71.6.2295
Abstract
The purification of a growth factor from bovine pituitary glands that controls the division of an ovarian cell line maintained in tissue culture is reported. Extraction of bovine pituitary glands at pH 4.5 followed by ammonium sulfate precipitation, chromatography on carboxymethylcellulose, and gel filtration on Sephadex G-50 gives yields of ovarian growth factor of about 7 mg/kg of glands. Contamination by other pituitary hormones was not detected. The preparation obtained has been characterized with respect to biological activity, molecular weight, and amino-acid composition. The implication of this growth factor in the development of ovarian tumors is discussed.Keywords
This publication has 28 references indexed in Scilit:
- Preparation and Characterization of Plasma Membranes from Bovine Corpus LuteumJournal of Biological Chemistry, 1973
- Human somatomedians. Purification and some studies on their biological actions.1973
- Bovine luteal cells in tissue cultureExperimental Cell Research, 1972
- Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.1972
- A Pituitary Growth-Promoting Factor for Articular Chondrocytes in Monolayer CultureEndocrinology, 1972
- Stimulatory effects of gonadotropins on the formation of cyclic adenosine 3′,5′-monophosphate by porcine granulosa cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 1971
- Restoration of Contact-Inhibited Growth to Transformed Cells by Dibutyryl Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1971
- "Contact inhibition" of cell division in 3T3 cells.Proceedings of the National Academy of Sciences, 1968
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- IN VITRO BIOASSAY OF CORTICOTROPIN: MODIFICATION AND STATISTICAL TREATMENTEndocrinology, 1955