EFFECTS OF VARIOUS CHEMICALS INCLUDING BILE-ACIDS AND CHEMICAL CARCINOGENS ON THE INHIBITION OF METABOLIC COOPERATION

Abstract

The effects of various chemicals such as bile acids, a lectin, polyamines and chemical carcinogens on metabolic cooperation in the hypoxanthine-guanine phosphoribosyl transferase system [in Chinese hamster cells] were surveyed. Among bile acids, lithocholic acid, chenodeoxycholic acid and ursodeoxycholic acid inhibited metabolic cooperation whereas cholic acid, deoxycholic acid and dehydrocholic acid did not. There was a structure-activity relationship among these compounds for the inhibition of metabolic cooperation. The metabolic cooperation was not inhibited by such chemicals as phytohemagglutinin, 3 polyamines, 7,12-dimethylbenz[a]anthracene, N-methyl-N''-nitro-N-nitrosoguanidine, 4-nitroquinoline 1-oxide and 3 biphenyl compounds. These results should be useful in relation to studies on the mechanism of inhibition of metabolic cooperation and on the relationship between this inhibitory activity and the tumor-promoting activity of various chemicals.