Effect of Different Intrinsic Factor Preparations on Co60 Vit. B12 Uptake in Rat Liver Slices

Abstract

A hog intrinsic factor concentrate and crude gastric juice specimens, obtained from human, dog, hog, and rat, were tested for intrinsic factor activity by comparing their ability to correct the deficient liver uptake of Cobalt-60 vitamin B12 by a pernicious anemia patient, by their ability to bind vitamin B12, and by their ability to increase the uptake of Cobalt-60 vitamin B12 by a rat liver slice. Human and hog returned the liver concentration of vitamin B12 by a pernicious anemia patient to normal; rat only slightly improved the uptake and dog did not affect it. The vitamin B12 binding ability was greatest with hog intrinsic factor concentrate with human, dog, hog, and rat showing progressively less binding. In the rat liver slice technique, hog intrinsic factor concentrate showed the greatest activity with hog and dog showing less activity. Each preparation inhibited vitamin B12 uptake at higher concentrations. On the other hand, rat showed inhibition only as did human. However, inhibition was greater with human. By the techniques tested, there is no direct correlation in the results from the various tests.