An Electron Microscope Comparison of Plasma Membrane Vesicles from Meristematic and Mature Soybean Root Tissue
- 1 June 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 63 (6), 1191-1197
- https://doi.org/10.1104/pp.63.6.1191
Abstract
Plasma membrane vesicles were isolated from homogenates of meristematic and mature soybean root tissue by differential sucrose gradient centrifugation. Vesicles were positively identified by the phosphotungstic acid-chromic acid procedure (PACP). The two preparations were comparable in size class distribution, mitochondrial contamination, and per cent plasma membrane vesicles present. Purity levels were estimated to be greater than 75%. The specificity of PACP was observed for a variety of cell types from both regions. Some variability in PACP staining was offset by careful modulation of the stain protocol and was found to be independent of developmental stage in subcellular fractions. Patchy or discontinuous staining, observed in both intact tissue and in subcellular fractions from both regions, was found to be a function of stain time.This publication has 21 references indexed in Scilit:
- Analysis of the Distribution of Potassium-stimulated Adenosine Triphosphatase Activity in Soybean RootPlant Physiology, 1979
- The isolation of plasma membrane from protoplasts of soybean suspension culturesJournal of Cell Science, 1977
- The site of cellulose synthesis. Hormone treatment alters the intracellular location of alkali-insoluble beta-1,4-glucan (cellulose) synthetase activities.The Journal of cell biology, 1975
- β-Glucan Synthetases of Plasma Membrane and Golgi Apparatus from Onion StemPlant Physiology, 1974
- STRUCTURE AND STAINING CHARACTERISTICS OF MYOFIBER EXTERNAL LAMINAJournal of Histochemistry & Cytochemistry, 1973
- Membrane-bound Adenosine Triphosphatase Activities of Oat RootsPlant Physiology, 1973
- Phosphotungstic Acid-Chromic Acid as a Selective Electron-Dense Stain for Plasma Membranes of Plant CellsStain Technology, 1972
- The tridimensional ultrastructure of native collagenous fibrils, cytochemical evidence for a carbohydrate matrixJournal of Ultrastructure Research, 1971
- Correlation between Ion Fluxes and Ion-stimulated Adenosine Triphosphatase Activity of Plant RootsPlant Physiology, 1970
- A low-viscosity epoxy resin embedding medium for electron microscopyJournal of Ultrastructure Research, 1969