Separation and Concentration of Schizonts of Plasmodium falciparum by Percoll Gradients1
- 1 May 1983
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 30 (2), 367-370
- https://doi.org/10.1111/j.1550-7408.1983.tb02932.x
Abstract
A technique for the separation of schizonts of P. falciparum is described. The different stages of the asexual cell cycle of the parasite were positioned according to their density in a continuous gradient of Percoll. Young trophozoites coincided with erythrocytes in a broad band corresponding to densities from 1.075-1.100 g/ml; schizonts were concentrated at a density approximating 1.062 g/ml. The viability of the parasites was unimpaired by this procedure. Young trophozoites and schizonts continued their normal life cycle when cultured after the separation procedure. The percentage of recovery was high, reaching 80% of the initial quantity. Possible applications of the technique are discussed.This publication has 11 references indexed in Scilit:
- Effects of Ca++ Depletion on the Asexual Cell Cycle of Plasmodium FalciparumThe American Journal of Tropical Medicine and Hygiene, 1982
- Plasmodium yoelii and Plasmodium berghei: Isolation of infected erythrocytes from blood by colloidal silica gradient centrifugationExperimental Parasitology, 1980
- A simple method for separation of uninfected erythrocytes from those infected withPlasmodium berghei and for isolation of artificially released parasitesZeitschrift Fur Parasitenkunde-Parasitology Research, 1980
- Synchronization of Plasmodium falciparum Erythrocytic Stages in CultureJournal of Parasitology, 1979
- Separation of viable schizont-infected red cells ofPlasmodium falciparumfrom human bloodPathogens and Global Health, 1978
- Schizont-Infected Cell Enrichment in Rodent MalariaJournal of Parasitology, 1976
- The preparation of malaria haemagglutination antigenPathogens and Global Health, 1976
- Human Malaria Parasites in Continuous CultureScience, 1976