Regulation of interferon receptor expression in human blood lymphocytes in vitro and during interferon therapy.
Open Access
- 1 May 1986
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 77 (5), 1632-1638
- https://doi.org/10.1172/jci112480
Abstract
Interferons (IFN) elicit antiviral and antineoplastic activities by binding to specific receptors on the cell surface. The binding characteristics of IFN to human lymphocytes were studied using IFN alpha 2 labeled with 125I to high specific activity. The specific binding curves generated were analyzed by the LIGAND program of Munson and Rodbard to determine receptor numbers. The number of receptors in peripheral blood lymphocytes (PBL) and tonsillar B-lymphocytes (TBL) from normal individuals were 505 +/- 293 (n = 10) and 393 +/- 147 (n = 3) respectively. When these cells were preincubated in vitro with unlabeled IFN alpha 2, the receptor number decreased to 82 +/- 45 and 61 +/- 16 respectively. Receptor binding activities recovered gradually over a period of 72 h when the cells were incubated in IFN-free medium. This recovery of receptors could be blocked by the addition of actinomycin D to the incubation medium. A similar decrease in receptor expression was observed in vivo in PBL from patients being treated daily with 5 X 10(6) units/m2 per d of IFN alpha 2 by subcutaneous injection, for acute lymphoblastic leukemia or papilloma virus infections. Receptor numbers in PBL in vivo were further reduced concurrent with the progression of IFN therapy. Thus the reduction in IFN receptor expression observed in vitro can be demonstrated in vivo. These studies indicate that monitoring IFN receptor expression in vivo can provide information regarding the availability of IFN receptors at the cell surface for the mediation of IFN actions during the course of IFN therapy.This publication has 30 references indexed in Scilit:
- Studies of the interferon receptorsPharmacology & Therapeutics, 1984
- Interferon receptor interaction. Internalization of interferon alpha2 and modulation of its receptor on human cellsEuropean Journal of Biochemistry, 1984
- Alpha Interferon for Induction of Remission in Hairy-Cell LeukemiaNew England Journal of Medicine, 1984
- Persistent Polyclonal Lymphocytosis of B LymphocytesNew England Journal of Medicine, 1982
- Evidence that types I and II interferons have different receptorsNature, 1981
- Interferon and spontaneous cytotoxicity in man. V. Enhancement of spontaneous cytotoxicity in patients receiving human leukocyte interferonInternational Journal of Cancer, 1980
- Epidermal Growth Factor1979
- Human Leukocyte Interferon for the Treatment of Herpes Zoster in Patients with CancerNew England Journal of Medicine, 1978
- ADMINISTRATION OF HUMAN FIBROBLAST INTERFERON IN CHRONIC HEPATITIS-B INFECTIONThe Lancet, 1976
- Effect of Prolonged Interferon Treatment on Mouse Embryonic Fibroblasts Transformed by Murine Sarcoma VirusJournal of General Virology, 1970