Protein Prosthesis: A Nonnatural Residue Accelerates Folding and Increases Stability
- 30 May 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (25), 7500-7501
- https://doi.org/10.1021/ja0351239
Abstract
Nonnatural residues can endow proteins with desirable properties. Here, replacing a proline residue that has a cis peptide bond in native ribonuclease A with 5,5-dimethyl-l-proline is shown to accelerate protein folding by 6-fold and enhance conformational stability by ΔTm = 2.8 ± 0.3 °C while having no effect on enzymatic activity. The rational use of this and other prosthetic segments could enable chemotherapeutic proteins to survive longer in vivo or retain activity after oral administration.Keywords
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