The Responses in Culture of Human Tumour Astrocytes and Neuroblasts to N6,O2′-Dibutyryl Adenosine 3′,5′-Monophosphoric Acid
Open Access
- 1 November 1972
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 11 (3), 639-667
- https://doi.org/10.1242/jcs.11.3.639
Abstract
N6, O2'-dibutyryl cyclic AMP (DBcAMP) (3 x 10-4 M) causes reproducible changes in the morphology, growth rate and terminal cell density of 3 different types of human tumour cells grown in culture. Human tumour astrocyte lines 1181N1 and EH-118MG show generalized ablation of membrane systems when grown in the presence of this cyclic AMP analogue. This is not consistent with differentiation; it may be a toxic effect but possibly represents the response of these cell types to DBcAMP. These cells also grow more slowly and to lower terminal cell densities as a function of the concentration of DBcAMP. Although the growth kinetics demonstrate a return of these malignant cells to a state of density-dependent growth, micrographic analyses of inter-cellular relationships reveal that the cells still grow in overlapping patterns. Thus as judged by the commonly accepted criteria, DBcAMP does not induce contact inhibition of growth. All observed changes induced by the presence of DBcAMP are rapidly and completely reversed upon its removal from the growth medium. Human neuroblasts (NB1) grown in the presence of DBcAMP show irreversible morphological changes and the rate and extent of cell division is reduced. There is marked hyperplasia of intracytoplasmic microfilaments, which is considered consistent with differentiation.Keywords
This publication has 34 references indexed in Scilit:
- Adenyl cyclase, phosphodiesterase and cyclic AMP dependent protein kinase of malignant glial cells in cultureLife Sciences, 1971
- Cyclic AMP levels in fibroblasts: Relationship to growth rate and contact inhibition of growthBiochemical and Biophysical Research Communications, 1971
- MICROFILAMENTS AND CELL LOCOMOTIONThe Journal of cell biology, 1971
- The influence of normal cells on the proliferation of tumour cells in cultureExperimental Cell Research, 1970
- ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATEThe Journal of cell biology, 1968
- Cyclic AMPAnnual Review of Biochemistry, 1968
- Effects of derivatives of adenosine 3′,5′-phosphate on liver slices and intact animalsBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Preparation of derivatives of adenosine 3′,5′-phosphateBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Antagonistic action of metabolic inhibitors on dibutyryl cyclic 3′5′-adenosine monophosphate-stimulated and caffeine-stimulated lipolysis in vitroLife Sciences, 1967
- The serial cultivation of human diploid cell strainsExperimental Cell Research, 1961