Parasexual Cycle in Cultivated Human Somatic Cells
- 7 November 1969
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 166 (3906), 761-763
- https://doi.org/10.1126/science.166.3906.761
Abstract
Somatic segregation for three different autosomes was demonstrated in two strains of human diploid fibroblasts derived from subjects known to be heterozygous for chromosomal variants. Recombinant diploid cells appeared within cultures of tetraploid clones isolted from mass cultures. Tetraploid cells regularly occur in mass cultures and within clones of diploid cells. Such a parasexual cycle (2n→4n→2n), with recombination of entire linkage groups, could from the basis of a beginning formal genetic analysis in man.Keywords
This publication has 7 references indexed in Scilit:
- Establishment of Human Skin Fibroblast Cultures with an Automatic Wedge Biopsy Instrument**From the Medical Instrument Facility and the Department of Pathology, University of Washington School of Medicine, Seattle, Washington.Journal of Investigative Dermatology, 1966
- A Definitive Cloning Technique for Human Fibroblast Cultures.Experimental Biology and Medicine, 1966
- Cytologic and genetic evidence of somatic segregation in mammals, birds, and fishesIn Vitro Cellular & Developmental Biology - Plant, 1966
- Interspecific somatic hybridsIn Vitro Cellular & Developmental Biology - Plant, 1966
- The limited in vitro lifetime of human diploid cell strainsExperimental Cell Research, 1965
- Alkaline phosphatase of human skin fibroblast culturesExperimental and Molecular Pathology, 1964
- Use of Tris(Hydroxymethyl)Aminomethane Buffers in Cultures of Diploid Human Fibroblasts.Experimental Biology and Medicine, 1964