Assessment of metal extract toxicity on human lymphocytes culturedin vitro
- 1 June 1996
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 31 (2), 183-191
- https://doi.org/10.1002/(sici)1097-4636(199606)31:2<183::aid-jbm4>3.0.co;2-j
Abstract
In this study the toxic effects of chromium, nickel, and cobalt extracts on in vitro cultured lymphocytes were evaluated. Graphite furnace atomic absorption spectrometry was used to measure the ion concentration. After serial dilution of the extracts, the viability of lymphocytes at 24, 48, and 72 h was estimated by flow cytometry, including propidium iodide staining and light scatter property assessment, and by MTT reduction test. The results of the investigation allowed us to conclude that 1) standardization of the procedure for preparing extracts is fundamental to obtaining repeatability of results; 2) the toxicity of an extract cannot be evaluated with a single viability assay; a combination of functional and structural tests is required; 3) when methods based on enzymatic reactions are performed, e.g. MTT test, it is advisable to replace the extract containing metal ions with fresh medium in order to avoid any interference with viability testing; 4) the amount of Co and Ni in the extract is similar, but the Cr release is very poor; 5) the lower toxicity of Cr extract probably is due to the lower ion concentration; 6) the assessment of 50% cytotoxic concentration, (TC50) allows quantification of materials toxicity and comparison of various metals; and 7) the determination of a noncytotoxic concentration, i.e., a concentration lower than TC10, is required for subsequent investigation of cell functions because such studies can be carried out only on viable cell population. © 1996 John Wiley & Sons, Inc.Keywords
This publication has 23 references indexed in Scilit:
- Uptake of metal cations by fibroblasts in vitroJournal of Biomedical Materials Research, 1993
- Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: A further pitfall in the use of the MTT assay for evaluating cell growth and survivalEuropean Journal Of Cancer, 1993
- Human cell culture studies with dental metallic materialsBiomaterials, 1992
- Storage and elimination of titanium, aluminum, and vanadium salts, in vivoJournal of Biomedical Materials Research, 1992
- Decreased neutrophil respiratory burst on exposure to cobalt‐chrome alloy and polystyrene in vitroJournal of Biomedical Materials Research, 1992
- Features of apoptotic cells measured by flow cytometryCytometry, 1992
- Synovial macrophage response to aluminium oxide ceramic and cobalt-chrome alloy wear particles in ratsBiomaterials, 1988
- Release of cobalt and nickel from a new total finger joint prosthesis made of vitalliumJournal of Biomedical Materials Research, 1983
- The action of cobalt, nickel and chromium on phagocytosis and bacterial killing by human polymorphonuclear leucocytes; its relevance to infection after total joint arthroplastyBiomaterials, 1983
- Metal ion release after total hip replacementBiomaterials, 1980