Anaphylaxis in Guinea Pig: Improbability of Release of Serotonin in the Schultz-Dale Reaction.

Abstract

The ilia and uteri of guinea pigs sensitized to egg albumin or bovine serum albumin were tested by the Schultz-Dale reaction to determine whether or not the reaction could be blocked with antiserotonic drugs. No inhibition of the reaction occurred in the pressence of bromlysergic acid diethylamide (BOL) or lysergic acid diethylamide (LSD); the reaction was modified by yohimbine, gramine and bufotenine, but the reaction to histamine was decreased in parallel to the decrease of reactivity to antigen. It was not possible to demonstrate the release of serotonin from sensitized guinea pig lung, uterus or ileum by the addition of antigen, in the pres-ence of the highly serotonin sensitive mouse uterus; nor was it possible, in the presence of BOL or LSD, to diminish the positive anaphylactic reaction of the histamine poisoned tissue strip. It was concluded that the release of serotonin in the Schultz-Dale reaction in the guinea pig was improbable.