In Vivo Determination of the Pyridine Nucleotide Reduction Charge by Carbon-13 Nuclear Magnetic Resonance Spectroscopy
- 7 October 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 222 (4619), 62-65
- https://doi.org/10.1126/science.6353573
Abstract
An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.This publication has 17 references indexed in Scilit:
- The Study of the Metabolism of 13C Labeled Substrates by 13C NMR Spectroscopy of Intact Cells, Tissues, and OrgansPublished by American Chemical Society (ACS) ,1982
- Phosphorus-31 nuclear magnetic resonance studies of bioenergetics in wild type and ATPase- Escherichia coli cellsBiochemistry, 1982
- Evidence suggesting that the NADPH/NADP ratio modulates the splitting of the isocitrate flux between the glyoxylic and tricarboxylic acid cycles, in Escherichia coliFEBS Letters, 1979
- Cellular Applications of 31 P and 13 C Nuclear Magnetic ResonanceScience, 1979
- The synthesis of nicotinic acid‐7‐13CJournal of Labelled Compounds and Radiopharmaceuticals, 1978
- GLYCEROL DISSIMILATION AND ITS REGULATION IN BACTERIAAnnual Review of Microbiology, 1976
- Quantitative analysis of carbon-carbon coupling in the carbon-13 nuclear magnetic resonance spectra of molecules biosynthesized from carbon-13-enriched precursorsJournal of the American Chemical Society, 1975
- Observation of tissue metabolites using 31P nuclear magnetic resonanceNature, 1974
- Biological probes. II. Ring labeled nicotinamideThe Journal of Organic Chemistry, 1974
- The pyridine nucleotide cycle: Presence of a nicotinamide mononucleotide-specific glycohydrolase in EscherichiacoliBiochemical and Biophysical Research Communications, 1972