Separation of viable microfilariae free of blood cells on Percoll gradients
- 1 March 1984
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Helminthology
- Vol. 58 (1), 69-70
- https://doi.org/10.1017/s0022149x00028078
Abstract
A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25M sucrose. The recovery of the microfilariae was 85 to 97%.This publication has 4 references indexed in Scilit:
- Combination of techniques for concentration and identification of microfilariae from peripheral bloodTransactions of the Royal Society of Tropical Medicine and Hygiene, 1981
- Immune reactions in human filariasisJournal of Clinical Microbiology, 1978
- A technique for isolating and concentrating microfilariae from peripheral blood by gradient centrifugationTransactions of the Royal Society of Tropical Medicine and Hygiene, 1975