PARALLEL BIOASSAY OF BOMBESIN AND LITORIN, A BOMBESIN‐LIKE PEPTIDE FROM THE SKIN OF Litoria aurea

Abstract

1 The spectrum of biological activity exhibited by litorin, a bombesin-like nonapeptide found in extracts of the skin of the Australian leptodactylid frog Litoria aurea was compared with that exhibited by the tetradecapeptide bombesin. 2 Litorin proved to be more potent than bombesin on isolated smooth muscle preparations and on the urinary bladder in situ. However, it was less potent on dog systemic blood pressure and kidney vasculature, activation of the renin-angiotensin system being slight or lacking. 3 Gastrin release and acid secretion produced by litorin was more rapid in onset but less intense and less sustained than that elicited by bombesin. The same could be observed for pancreatic secretion. 4 Gall bladder contraction stimulated by litorin was probably caused by a double action of the peptide, directly on the bladder smooth muscle, and indirectly by cholecystokinin release. 5 In its effects on the myo-electric activity of the dog duodenum (inhibition of spikes and increase in frequency of pacesetter potentials leading to the appearance of a sequence of slow and small potentials) litorin possessed approximately 50 to 70% of the activity of bombesin.