CHARACTERIZATION OF A NEWLY ESTABLISHED HUMAN GASTRIC-CANCER CELL-LINE HGT-1 BEARING HISTAMINE H-2-RECEPTORS

Abstract

A human gastric adenocarcinoma cell line, HGT-1, was established in vitro from the primary tumor of a 60 yr old patient. Histological examination of the tumor revealed a poorly differentiated adenocarcinoma. Primary tumor cells were cloned in soft agarose and gave rise to tumor colonies. The procedures to form a continuous cell line from the agarose colonies are described. The cultured cells grew as monolayers of closely apposed polygonal cells with a population-doubling time of 19.48 .+-. 1.20 (SE) h during exponential growth at passage 59. They had an epithelial morphology. Ultrastructural studies revealed the presence of microvilli and tight junctions. The HGT-1 cell line is tumorigenic in nude mice and has a hyperdiploid karyotype with a modal number of 57 chromosomes. It exhibits numerous marker chromosomes. These human gastric epithelial cells do not secrete mucus or carcinoembryonic antigen. They exhibit functional histamine H2-receptors mediating cellular cAMP production and adenylate cyclase activation. The use of a soft-agarose clonogenic assay permitted development of a cancer cell line without the problems of fibroblastic cell contamination. The existence of histamine H2-receptors on gastric HGT-1 cells stresses the importance of this line as a model for studies of regulatory mechanisms involved in gastric secretion.