A SPECIFIC HYDROXYLAMINE-FERRIC CHLORIDE REACTION FOR HISTOCHEMICAL LOCALIZATION OF PECTIN
- 1 January 1959
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 34 (4), 209-211
- https://doi.org/10.3109/10520295909114676
Abstract
An alkaline solution of hydroxylamine is caused to react with the methyl esters of pectin present in sections of plant tissues to produce hydroxamic acids which, after acidification with hydrochloric acid, yield a red color upon addition of 2.5% ferric chloride solution. The test is specific and preferable to the use of ruthenium red for localization of pectin in the compound middle lamella. Subjecting sections to methylation and using reagents made up in 60% alcohol increases the intensity and sharpness of the color formation.Keywords
This publication has 3 references indexed in Scilit:
- Histological and Histochemical Changes in Developing and Ripening Peaches. II. The Cell Walls and PectinsAmerican Journal of Botany, 1959
- Measurement of Plant Pectic Substances, Reaction of Hydroxylamine with Pectinic Acids. Chemical Studies and Histochemical Estimation of the Degree of Esterification of Pectic Substances in FruitJournal of Agricultural and Food Chemistry, 1959
- Use of the Hydroxamic Acid Reaction for Determining Pectinesterase ActivityStain Technology, 1958