Preparation and characterization of macroporous chitosan–gelatin/β‐tricalcium phosphate composite scaffolds for bone tissue engineering

Abstract

A biodegradable composite scaffold was developed using β-tricalcium phosphate (β-TCP) with chitosan (CS) and gelatin (Gel) in the form of a hybrid polymer network (HPN) via co-crosslinking with glutaraldehyde. Various types of scaffolds were prepared by freezing and lyophilizing. These scaffolds were characterized by Fourier transform infrared, X-ray diffractometer, and scanning electron microscopy. The macroporous composite scaffolds exhibited different pore structures. Compressive properties were improved, especially compressive modulus from 3.9–10.9 MPa. Biocompatibility was evaluated subcutaneously on rabbits. A mild inflammatory response was observed over 12 weeks. The results suggest that the scaffolds can be utilized in nonloading bone regeneration. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 844–855, 2003