Induction of Transplantable Liver Tumors in DBF1 Mice After Oral Administration of N,N′-2,7-Fluorenylenebisacetamide2

Abstract

The carcinogenic effect of N,N′-2,7-fluorenylenebisacetamide (2,7-FAA) was studied in 56 (DBA/2 ♂ × C57BL/6 ♀)F₁ (DBF₁) mice fed a diet containing 0.025% 2,7-FAA for 3–5 months. Adenoma of the liver was found in 2 mice, carcinoma of the liver in 49, leukemia in 3, and adenomatous hyperplasia in 3. Lung metastasis from the liver carcinoma was observed in 2 animals. Several liver carcinomas were successfully transplanted subcutaneously and retained their original character in the fifth transplant generation. In the present experiment, despite the fact that the total amount of 2,7-FAA ingested by female mice was less than that by the male mice, there was no difference in the incidence of liver tumors. The adenomatous hyperplasia of the glandular stomach was seen only in the male mice surviving over 360 experimental days.