SUMMARY: The carotenoids of Sarcina flava were examined; four main fractions were separated. These were hydrocarbons, mono- and di-hydroxylated compounds and a very polar fraction. Further separation of the hydrocarbon fraction showed the presence of nine compounds and of the very polar fraction which showed an all-trans form and three cis isomers. Data for these fractions is presented; at least two of the fractions are C50 carotenoids.