THE IN VITRO GROWTH OF ACER SACCHARUM AND ACER PENNSYLVANICUM CALLUS TISSUE

Abstract

The media and conditions for the growth of isolated maple tissues were determined. Tissues isolated from Acer pennsylvanicum and Acer saccharum were grown on a medium containing 25% coconut milk, 2 p.p.m. naphthalene-acetic acid, 2% sucrose, and 2 times the salt concentrations suggested by White and Nitsch. The cultures have maintained an actively growing condition in the dark at 26–27 °C. The utilization of various carbohydrates by A. pennsylvanicum tissue was determined. Sucrose, glucose, and raffinose were utilized most efficiently. Extracellular starch digestion was detected using isolated A. saccharum stem callus.