Oocyst age and excystation of Cryptosporidium parvum
- 1 June 1986
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 64 (6), 1254-1255
- https://doi.org/10.1139/z86-187
Abstract
The effects of temperature, tap water, and excysting fluid were tested on oocysts of Cryptosporidium parvum that had been stored for 14, 32, or 43 weeks at 4 °C. In preparations containing 14-week-old oocysts, sporozoites excysted in the presence of excysting fluid at 37 °C, but did not excyst in excysting fluid at 22 °C nor in tap water at 22 or 37 °C. In contrast, numerous empty oocysts but few or no sporozoites were present in preparations of 32- or 43-week-old oocysts incubated in excysting fluid or tap water at 22 or 37 °C. Sporozoites in tap water lysed soon after leaving the oocyst. Thus, sporozoites of C. parvum will excyst from young oocysts only in the presence of excysting fluid at 37 °C, whereas older oocysts deteriorate with an increase in age and apparently become susceptible to the harmful effects of a hypotonic environment.This publication has 1 reference indexed in Scilit:
- The Effects of Reducing Conditions, Medium, pH, Temperature, and Time on in Vitro Excystation of CryptosporidiumThe Journal of Protozoology, 1984