Excised intestinal strips from the duodenum, ileum, and colon were suspended in oxygenated Locke''s solution at 38[degree]C. Levers were arranged which recorded rate and amplitude of rhythmic contractions and tonus waves, and time during which the strips exhibited spontaneous contractions. Tracings were run from refrigerated segments at 1, 2, 3, and 4 days after death, with tracings from normal animals as controls. Absence of vitamin B from the diet of a rat diminished the time during which an excised strip exhibited spontaneous contractions. Amplitude and rate of rhythmic contraction were markedly decreased in the spastic stage of beriberi rats. The tonus of the intestinal musculature was also lessened. Segments from the beriberi intestine placed on ice for 24 hrs. showed no spontaneous contraction or tone, whereas normal strips were active on the 2nd day. Constipation and decreased motility of intestinal tract are characteristic of beriberi rats. In guinea pigs deprived of vitamin C, the duration or spontaneous activity of the strip excised during the moribund stage was decreased. There was also diminution in number of rhythmic contractions, except in the ileum, where the reverse was true. Increased amplitude of rhythmic contractions indicated increased motility in the colon and slight hyperactivity in the duodenal strip. In general, tonus of the intestinal wall was considerably increased in the scorbutic segment.