Isolation and Characterization of Glycosaminoglycans from the Furth Murine Mastocytoma

Abstract

New methods for isolation and fractionation by partition are described and compared with existing techniques. Substantially purer products were isolated by partition as compared to precipitation with hexadecylpyridinium chloride. The glyco-saminoglycans isolated from Furth murine mastocytoma tumor were found to consist of 78–80% heparin, 12–13% chondroitin sulfate, and 8–9% hyaluronate. Dermatan sulfate was not detected. Two heparin-like glycosaminoglycans could be isolated by partition fractionation in the phase system 1-butanol/aqueous NaCl containing hexadecylpyridinium chloride. The composition of one was typical of heparins. However, the other glycosaminoglycan contained only 0.47 moles N-sulfate/mole uronate, but had electrophoretic and partition properties characteristic of heparin.