IMPAIRMENT OF ISOPROTERENOL, H-2 HISTAMINE, AND PROSTAGLANDIN-E, RESPONSE OF HUMAN-GRANULOCYTES AFTER INCUBATION INVITRO WITH LIVE INFLUENZA VACCINES

Abstract

The release of the lysosomal enzyme .beta.-glucuronidase from granulocytes follows incubation in vitro with complement-activated zymosan particles. Release of .beta.-glucuronidase is inhibited by isoproterenol, histamine and prostaglandin E1. The effect of incubating a live, bivalent (A + B) influenza vaccine on the granulocyte response to the agonists described was studied. After incubation in vitro with the live, bivalent influenza vaccine, there was a significantly impaired granulocyte response to all 3 agonists. The change in the response of polymorphonuclear leukocytes to isoproterenol was similar to an impairment in .beta.-adrenergic response found during respiratory infections in vivo. The viral-induced changes in the granulocyte response to isoproterenol may reflect similar alteration in other tissues such as variable control of the airways and provide 1 explanation for the occurrence of airway dysfunction during respiratory infections.