Abstract
The experimental evidence indicates that carotenoid-pigmented microorganisms are not essential for the growth of the cellular slime mold A. rosea. Certain non-pigmented Gram-negative bacteria, in pure culture or combined with the colorless yeast Hansenula anomala, supported the growth of A. rosea on cornmeal-dextrose agar supplemented with 0.1% yeast extract. The addition of cell-free filtrates from 2 of these bacteria or from the pink yeast Rhodotorula mucilaginosa also enabled Acrasis to grow on Hansenula. In the absence of these supplements, H. anomala does not support Acrasis growth.

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