Effect of room fluorescent light on the deterioration of tissue culture medium
- 1 January 1976
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 12 (1), 19-22
- https://doi.org/10.1007/bf02832788
Abstract
A major cause of tissue culture medium deterioration is exposure to room fluorescent light. Riboflavin and tryptophan present in Dulbecco's modified Eagle's minimum essential medium, when exposed to light, yield toxic photoproducts responsible for loss of the ability of the medium to support clonal growth of human, mouse and Chinese hamster cell lines. Procedures for minimizing medium deterioration are discussed.Keywords
This publication has 7 references indexed in Scilit:
- LETHAL EFFECT OF “DAYLIGHT” FLUORESCENT LIGHT ON HUMAN CELLS IN TISSUE‐CULTURE MEDIUMPhotochemistry and Photobiology, 1975
- Effect of Near-Ultraviolet and Visible Light on Mammalian Cells in Culture II. Formation of Toxic Photoproducts in Tissue Culture Medium by BlacklightProceedings of the National Academy of Sciences, 1974
- Lethal Effect of Near-ultraviolet Irradiation on Mammalian Cells in CultureNature, 1974
- A New Reduced Human-Mouse Somatic Cell Hybrid Containing the Human Gene for Adenine PhosphoribosyltransferaseProceedings of the National Academy of Sciences, 1971
- Radiation Response of Mammalian Cells Grown in Culture: I. Repair of X-Ray Damage in Surviving Chinese Hamster CellsRadiation Research, 1960
- Plaque production by the polyoma virusVirology, 1959