Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance
Open Access
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (24), 6081-6097
- https://doi.org/10.1093/nar/8.24.6081
Abstract
The DNA sequence of the ermC gene of plasmid pE194 is presented. This determinant is responsible for erythromycin-induced resistance to the macrolide-lincosamide-streptogramin B group of antibiotics and specifies a 29,000 dalton inducible protein. The locations of the ermC promoter, as well as that of a probable transcriptional terminator, are established both from the sequence and by transcription mapping. The sequence contains an open reading frame sufficient to encode the previously identified 29,000 dalton ermC protein. Between the promoter and the putative ATG start codon is a 141 base pair leader sequence, within which several regulatory (constitutive) mutations have been mapped and sequenced. The leader has a second open reading frame, sufficient to encode a 19 amino acid peptide. It is suggested that induction by erythromycin involves a shift between alternative ribosome-bound mRNA conformations, so that the ribosome binding sequence and the start codon for synthesis of the 29K protein are unmasked in the presence of inducer. Possible active and inactive folded configurations of the leader sequence are presented, as well as the effects on these configurations of regulatory mutations.Keywords
This publication has 33 references indexed in Scilit:
- Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenylphosphoryl-uridlne-2′(3′)-phosphateNucleic Acids Research, 1977
- A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: Some modifications in the sanger method for RNA sequencingJournal of Molecular Evolution, 1976
- Three distinct plasmids originating in the same Staphylococcus aureus strain.1976
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- Determinant of cistron specificity in bacterial ribosomesNature, 1975
- The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding SitesProceedings of the National Academy of Sciences, 1974
- Alteration of 23 S ribosomal RNA and erythromycin-induced resistance to lincomycin and spiramycin in Staphylococcus aureusJournal of Molecular Biology, 1973
- Structure of an inducibly methylatable nucleotide sequence in 23S ribosomal ribonucleic acid from erythromycin-resistant Staphylococcus aureusBiochemistry, 1973
- Altered Methylation of Ribosomal RNA in an Erythromycin-Resistant Strain of Staphylococcus aureusProceedings of the National Academy of Sciences, 1971
- The Effect of Antibiotics on the Coded Binding of Peptidyl‐tRNA to the Ribosome and on the Transfer of the Peptidyl Residue to PuromycinEuropean Journal of Biochemistry, 1969