Hypersensitive resistance. Isolation and characterization of glycoproteins from plants with localized infections

Abstract

Local lesions were induced on Phaseolus vulgaris var. Pinto by inoculation with tobacco necrosis virus. At 24–36 and 120 h, alkali-soluble protein–polysaccharide complexes (I and II, respectively) were isolated from the primary leaves.Amino acid analyses indicated that the protein components of I and II were similar and related in composition to known plant cell wall proteins. The two preparations differed principally in their carbohydrate moieties; preparation II contained more glucose and this glucose was shown to occur as a β-(1 → 4)-linked glucan. Preparation I contained mostly (1 → 4)-linked glucose but, in addition, (1 → 3)-linked glucose (callose-type linkage) was present. Both complexes contained acid-labile arabinose as well as mannose and galactose.